095 Single-cell Profiling Reveals a Highly Specific, Compartmentalized Functional Response in the Cutaneous Immune System
نویسندگان
چکیده
منابع مشابه
Highly multiplexed profiling of single-cell effector functions reveals deep functional heterogeneity in response to pathogenic ligands.
Despite recent advances in single-cell genomic, transcriptional, and mass-cytometric profiling, it remains a challenge to collect highly multiplexed measurements of secreted proteins from single cells for comprehensive analysis of functional states. Herein, we combine spatial and spectral encoding with polydimethylsiloxane (PDMS) microchambers for codetection of 42 immune effector proteins secr...
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G-protein-coupled receptor (GPCR) expression is extensively studied in bulk cDNA, but heterogeneity and functional patterning of GPCR expression in individual vascular cells is poorly understood. Here, we perform a microfluidic-based single-cell GPCR expression analysis in primary smooth muscle cells (SMC) and endothelial cells (EC). GPCR expression is highly heterogeneous in all cell types, wh...
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Paecilomyces Sp. are saprophytic fungi, which have rarely been pathogen for human. Herein, we report a case of cutaneous infection with Paecilomyces lilacinus with unusual presentations in a healthy young woman. A biopsy provided an initial diagnosis of fungal elements in the tissue. Multiple positive fungal cultures were obtained from the biopsied tissue. Microscopic and macroscopic examinatio...
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 2020
ISSN: 0022-202X
DOI: 10.1016/j.jid.2020.03.097